NH-BSRS - NMDG-HEPES Brain Slice Recovery Solution (1000 ml)
NH-BSRS - NMDG-HEPES Brain Slice Recovery Solution (1000 ml)
NMDG-HEPES Brain Slice Recovery Solution (1000 ml) (NH-BSRS) is used for recovery of acute... mehr
Produktinformationen "NH-BSRS - NMDG-HEPES Brain Slice Recovery Solution (1000 ml)"
NMDG-HEPES Brain Slice Recovery Solution (1000 ml) (NH-BSRS) is used for recovery of acute brain slices from rats and mice after the cutting procedure.
Product information
Ready to Use NH-BSRS solution
autoclaved
Instructions for use:
Ready to use:
For 1000 ml NH-BSRS Solution:
1. Oxygenate 500 ml Base A for 10 min.
2. Add slowly 500 ml Base B
3. Oxygenate NH-BSRS Solution during use
pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)
Use immediately once opened. Store at 4 - 8 °C
Ional composition of MH-BSCS Solution
N-Methyl-D-Glucamine = 93 mM
NaHCO3 = 30 mM
Glucose = 25 mM
Na L-Ascorbate = 5 mM
MgSO4 = 10 mM
NaH2PO4 = 1,25 mM
KCl = 2,5 mM
CaCl2 = 0,5 mM
HEPES = 20 mM
Thiourea = 2 mM
Na Pyruvate = 3 mM
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