MH-BSCS - Modified HEPES Brain Slice Cutting Solution (1000 ml)

Modified HEPES Brain Slice (1000 ml) (MH-BSCS) is used for cutting, recovery and storage of acute brain slices from rats and mice.

Product information

• Ready to Use MH-BSCS solution

• autoclaved

Instructions for use:

Ready to use:

For 1000 ml MH-BSCS Solution:

1. Oxigenate 500 ml Base A for 10 min.

2. Add slowly 500 ml Base B

3. Oxygenate MH-BSCS Solution during use

pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)

Use immediately once opened. Store at 4 - 8 °C

Ional composition of MH-BSCS Solution

NaCl = 92 mM

NaHCO3 = 30 mM

Glucose = 25 mM

Na L-Ascorbate = 5 mM

MgSO4 = 10 mM

NaH2PO4 = 1,25 mM

KCl = 2,5 mM

CaCl2 = 0,5 mM

HEPES = 20 mM

Thiourea = 2 mM

Na Pyruvate = 3 mM