Normal Frog Ringer (NFR) is used for lab experiments with Xenopus laevis oocytes to guarantee a physiological environment with respect to homeostasis, pH and osmolarity.

Product information

• Ready to Use NFR solution or 10x concentrate available

• autoclaved

Instructions for use:

Ready to use:

Direct use

10x concentrate:

For 1000 ml NFR Solution:

1. Pour 100 ml NFR (10x) into a flask

2. Add deionized water to a final volume of 1000 ml

Adjust to pH 7.4

Use immediately once opened. Store at 4 - 8 °C

Ional composition of NFR Solution

NaCl = 90 mM

KCl = 2 mM

CaCl2 = 2 mM

MgCl2 = 1 mM

HEPES = 5 mM