Artificial cerebrospinal fluid (aCSF) is a biological buffer that enables a vital environment for neuronal tissue by maintaining the homeostasis, osmolarity, and pH at physiological levels. ACSF is commonly used for many lab experiments including in vitro and in vivo applications.

Product information

• Ready to Use ACSF solution or 20x concentrate available

• Carbogen (95% oxygen and 5% carbon dioxide) supply is necessary for proper Bicarbonate pH buffering.

• autoclaved

Instructions for use:

Ready to use:

For 1000 ml ACSF Solution:

1. Oxygenate 500 ml Base A for 10 min

2. Add slowly 500 ml Base B

3. Oxygenate ACSF Solution during use

20x concentrate:

For 1000 ml ACSF Solution:

1. Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water

2. Add slowly 50 ml Base B (20x)

3. Oxygenate ACSF Solution during use

pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)

Use immediately once opened. Store at 4 - 8 °C

Ional composition of ACSF Solution (Base A + Base B)

NaCl = 125 mM

KCl = 3 mM

CaCl2 = 2.5 mM

MgSO4 = 1.3 mM

NaH2PO4 = 1.25 mM

NaHCO3 = 26 mM

Glucose = 13 mM