ACSF - Artificial Cerebrospinal Fluid (1000 ml)

Product information

• Ready to Use ACSF solution or 20x concentrate available
• Carbogen (95% oxygen and 5% carbon dioxide) supply is necessary for proper Bicarbonate pH buffering.
• autoclaved

Instructions for use:

Ready to use: For 1000 ml ACSF Solution: 1. Oxygenate 500 ml Base A for 10?min 2. Add slowly 500 ml Base B 3. Oxygenate ACSF Solution during use

20x concentrate: For 1000 ml ACSF Solution: 1. Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water 2. Add slowly 50 ml Base B (20x) 3. Oxygenate ACSF Solution during use

pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene) Use immediately once opened. Store at 4 - 8 °C

Ional composition of ACSF Solution (Base A + Base B)