Cell & Tissue Slice Media for your lab work
Phosphate Buffered Saline (PBS) is used as isotonic buffer solution in biological research.Product informationReady to Use PBS solution or 10x concentrate availableautoclaved Instructions for use:Ready to use:Direct use10x concentrate:For 1000 ml PBS Solution:1. Pour 100 ml PBS (10x) into a flask 2. Add deionized water to a final volume of 1000 mlAdjust to pH 7.4Use immediately once opened. Store at 4 - 8 °CIonal composition of PBS SolutionNaCl = 137 mMNa2HPO4 = 10 mMKCl = 2,7 mMKH2PO4 = 2 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Inhalt: 0.5 Liter (52,36 € / 1 Liter)
Artificial cerebrospinal fluid (aCSF) is a biological buffer that enables a vital environment for neuronal tissue by maintaining the homeostasis, osmolarity, and pH at physiological levels. ACSF is commonly used for many lab experiments including in vitro and in vivo applications.Product informationReady to Use ACSF solution or 20x concentrate availableCarbogen (95% oxygen and 5% carbon dioxide) supply is necessary for proper Bicarbonate pH buffering.autoclaved Instructions for use:Ready to use:For 1000 ml ACSF Solution:1. Oxygenate 500 ml Base A for 10?min2. Add slowly 500 ml Base B3. Oxygenate ACSF Solution during use20x concentrate:For 1000 ml ACSF Solution:1. Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water 2. Add slowly 50 ml Base B (20x)3. Oxygenate ACSF Solution during usepH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)Use immediately once opened. Store at 4 - 8 °CIonal composition of ACSF Solution (Base A + Base B)NaCl = 125 mMKCl = 3 mMCaCl2 = 2.5 mMMgSO4 = 1.3 mMNaH2PO4 = 1.25 mMNaHCO3 = 26 mMGlucose = 13 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Normal Frog Ringer (NFR) is used for lab experiments with Xenopus laevis oocytes to guarantee a physiological environment with respect to homeostasis, pH and osmolarity. Product information Ready to Use NFR solution or 10x concentrate available autoclaved Instructions for use: Ready to use: Direct use 10x concentrate: For 1000 ml NFR Solution: 1. Pour 100 ml NFR (10x) into a flask 2. Add deionized water to a final volume of 1000 ml Adjust to pH 7.4 Use immediately once opened. Store at 4 - 8 °C Ional composition of NFR Solution NaCl = 90 mM KCl = 2 mM CaCl2 = 2 mM MgCl2 = 1 mM HEPES = 5 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Inhalt: 0.5 Liter (66,64 € / 1 Liter)
Modified Barth's Solution (MBS) is used forstorage and maintanance of oocytes from Xenopus laevis.Product informationReady to Use MBS solution or 10x concentrate availableautoclaved Instructions for use:Ready to use:Direct use10x concentrate:For 1000 ml MBS Solution:1. Pour 100 ml MBS (10x) into a flask 2. Add deionized water to a final volume of 1000 mlAdjust to pH 7.4Use immediately once opened. Store at 4 - 8 °CIonal composition of MBS SolutionNaCl = 88 mMKCl = 1 mMCaCl2 = 0,4 mMCa(NO3)2 = 0,33 mMMgSO4 = 0,8 mMTRIS-HCl = 5 mMNaHCO3 = 2,4 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Inhalt: 0.5 Liter (66,64 € / 1 Liter)
Modified HEPES Brain Slice (1000 ml) (MH-BSCS) is used for cutting, recovery and storage of acute brain slices from rats and mice.Product informationReady to Use MH-BSCS solutionautoclaved Instructions for use:Ready to use:For 1000 ml MH-BSCS Solution:1. Oxigenate 500 ml Base A for 10 min. 2. Add slowly 500 ml Base B3. Oxygenate MH-BSCS Solution during usepH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)Use immediately once opened. Store at 4 - 8 °CIonal composition of MH-BSCS SolutionNaCl = 92 mMNaHCO3 = 30 mMGlucose = 25 mMNa L-Ascorbate = 5 mMMgSO4 = 10 mMNaH2PO4 = 1,25 mMKCl = 2,5 mMCaCl2 = 0,5 mMHEPES = 20 mMThiourea = 2 mMNa Pyruvate = 3 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Modified Tyrode's Solution for use with mammalian heart tissue.Product informationReady to Use MTS solution or 20x concentrate availableCarbogen (95% oxygen and 5% carbon dioxide) supply is necessary for proper Bicarbonate pH buffering.autoclaved Instructions for use:Ready to use:For 1000 ml MTS Solution:1. Oxygenate 500 ml Base A for 10?min2. Add slowly 500 ml Base B3. Oxygenate ACSF Solution during use20x concentrate:For 1000 ml MTS Solution:1. Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water 2. Add slowly 50 ml Base B (20x)3. Oxygenate ACSF Solution during usepH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)Use immediately once opened. Store at 4 - 8 °CIonal composition of MTS Solution (Base A + Base B)NaCl = 126,7 mMKCl = 5,4 mMCaCl2 = 1,8 mMMgCl2 = 1,05 mMNaH2PO4 = 0,42 mMNaHCO3 = 22 mMGlucose = 5 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Calcium-free Modified Barth's Solution (CMBS) is used for defolliculation of Xenopus laevis oocytes by collagenase treatment.Product informationReady to Use CMBS solution or 10x concentrate availableautoclaved Instructions for use:Ready to use:Direct use10x concentrate:For 1000 ml CMBS Solution:1. Pour 100 ml CMBS (10x) into a flask 2. Add deionized water to a final volume of 1000 mlAdjust to pH 7.4Use immediately once opened. Store at 4 - 8 °CIonal composition of CMBS SolutionNaCl = 88 mMKCl = 1 mMMgSO4 = 0,8 mMTRIS-HCl = 5 mMNaHCO3 = 2,4 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Inhalt: 0.5 Liter (66,64 € / 1 Liter)
Choline Chloride Brain Slice Cutting Solution (CC-BSCS) is used for cutting acute brain slices from rats and mice.Product informationReady to Use CC-BSCS solutionautoclaved Instructions for use:Ready to use:For 1000 ml CC-BSCS Solution:1. Oxigenate 500 ml Base A for 10 min. 2. Add slowly 500 ml Base BpH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)Use immediately once opened. Store at 4 - 8 °CIonal composition of CC-BSCS SolutionCholine Chloride = 110 mMNaHCO3 = 25 mMGlucose = 25 mMNa L-Ascorbate = 11,6 mMMgSO4 = 7 mMNaH2PO4 = 1,25 mMKCl = 2,5 mMCaCl2 = 0,5 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Sodium-free High Surcrose Brain Slice Cutting Solution (SHS-BSCS) is used for cutting acute brain slices from rats and mice.Product informationReady to Use SHS-BSCS solutionautoclaved Instructions for use:Ready to use:For 1000 ml SHS-BSCS Solution:1. Oxygenate 500 ml Base A for 10 min2. Add slowly 500 ml Base B3. OxygenateSHS-BSCS Solution during usepH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)Use immediately once opened. Store at 4 - 8 °CIonal composition of SHS-BSCS SolutionSucrose = 234 mMNaHCO3 = 26 mMGlucose = 11 mMMgSO4 = 10 mMNaH2PO4 = 1,25 mMKCl = 2,5 mMCaCl2 = 0,5 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
High Sucrose Brain Slice Cutting Solution (HS-BSCS) is used for cutting acute brain slices from rats and mice.Product informationReady to Use HS-BSCS solutionautoclaved Instructions for use:Ready to use:For 1000 ml HS-BSCS Solution:1. Oxygenate 500 ml Base A for 10 min2. Add slowly 500 ml Base B3. Oxygenate HS-BSCS Solution during usepH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)Use immediately once opened. Store at 4 - 8 °CIonal composition of HS-BSCS SolutionSucrose = 75 mMNaHCO3 = 24 mMGlucose = 25 mMMgCl2 = 4 mMNaH2PO4 = 1,25 mMKCl = 2,5 mMCaCl2 = 0,5 mMNaCl = 85 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Modified Artificial Cerebrospinal Fluid (mACSF) is used for technical use Product information Ready to Use mACSF solution 500 ml - autoclaved Instructions for use: Ready to use: Direct use. Adjust to pH 7.4 Use immediately once opened. Store at 4 - 8 °C Ional composition of mACSF Solution NaCl = 152.25 mM KCl = 3 mM CaCl2 x 2H2O = 2.5 mM MgSO4 x 7H2O= 1.3 mM C6H12O6 x H2O = 13 mM HEPES = 5 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Inhalt: 0.5 Liter (69,02 € / 1 Liter)
Vitelline Removing Solution (VRS) is used for lab experiments with Xenopus laevis oocytes to enable removal of vitelline membrane from defolliculated Xenopus laevis oocytes for patch-clamp recordings. Product information Ready to Use VRS solution 500 ml - autoclaved Instructions for use: Ready to use: Direct use. After 5 minutes of treatment: Handle devitellinated oocytes with great care. Adjust to pH 7.2 Use immediately once opened. Store at 4 - 8 °C Ional composition of VRS Solution HO2CCH2CH(NH2)CO2K = 200 mM KCl = 20 mM MgCl2 = 1 mM EGTA = 10 mM HEPES = 10 mM Information For laboratory use only Manufacturer Dr. Lohmann Diaclean GmbH Oespeler Kirchweg 10 44379 Dortmund, Deutschland E-Mail: info@diacleanshop.com
Inhalt: 0.5 Liter (88,06 € / 1 Liter)